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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Clin Cancer Res. 2012 Oct 18;18(24):6690–6701. doi: 10.1158/1078-0432.CCR-12-0770

Figure 1. ATO/EA has synergistic effect on apoptosis in HL-60 and K562 cells.

Figure 1

(A & B) Quantification of apoptosis. HL-60 (A) and K562 (B) cells were treated for 24 h with a fixed ratio of ATO (0.5–4 μM) and EA (10–100 μM). Apoptotic cells were quantified by microscopic detection and counting of AO and EB-stained cells. (C & D) Combination index (CI) of ATO with EA on apoptosis induction in HL-60 (C) and K562 (D) cells. The nature of interaction between ATO and EA was characterized by median dose effect analysis using CompuSyn software. CI values less than 1.0 (horizontal line) corresponds to a synergistic interaction. Fa on the x-axis denotes the fraction affected (e.g. Fa of 0.5 is equivalent to a 50% apoptosis induction.)