Mdr2-deficient mice with HCC were treated with the Hh-inhibitor, GDC-0449, or vehicle (N=5 mice/group); effects on stroma of microdissected tumor nodules were evaluated by morphometry of immunostained sections and QRT-PCR. Representative sections and mean +/− SEM data are displayed (*p<0.05, **p<0.01 GDC-0449-vs. vehicle-treated groups). (A) Co-localization of PKM2 (green) with αSMA (brown) in tumor from vehicle-treated mouse (400x). (B) QRT-PCR for Gli2, αSma, Glut1, Hk2, Pgk1 in liver tumor mRNA. (C) Tumor sections from Vehicle- and GDC-0449 treated mice demonstrate (Top) αSMA, (Middle) PKM2, and (Bottom) MCT4 (brown, 200x)