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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Exp Hematol. 2012 Sep 25;41(1):102–112. doi: 10.1016/j.exphem.2012.09.007

FIG. 4.

FIG. 4

(A) Relative gene expression of Gpr171 and myeloid differentiation genes (Csfr1 and Mpo) in 32D cells transduced with anti-Gpr171 siRNAs. Cells were analyzed 48hrs post-transduction and mRNA levels were assessed by Real-Time PCR. (B) Percentages of Mac-1+ 32D cells after transduction with MSCV-Gpr171 or anti-Gpr171 siRNAs. Cells were analyzed by flow-cytomtry 2 and 5 days post-transduction. (C) Representative histograms of Mac-1 expression in 32D cells 96 hours after transduction with MSCV-Gpr171 or anti-Gpr171 siRNAs. (D) Mean Fluorescence Intensity (MFI) of myeloid marker Gr-1 in 32D cells transduced with MSCV-Gpr171 or anti-Gpr171 siRNAs. (E) 32D colony formation (espressed as CFU-Cs) is decreased after transduction with anti-Gpr171 siRNAs.