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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Exp Hematol. 2012 Sep 25;41(1):102–112. doi: 10.1016/j.exphem.2012.09.007

FIG. 5.

FIG. 5

Gpr171 over-expression in Sca-1+ cells in vitro. (A) Schematic representation of the experimental model utilized to study Gpr171 role in murine hematopoiesis. Sca-1+ HSCs/HPCs were collected from WT CD45.2 donor mice previously injected with 5-FU and transduced with the retroviral MSCV-Gpr171 vector or with the empty control vector. Transduced cells were then utilized for both in vitro and in vivo studies. (B) Percentages of GFP+, primary murine hematopoietic cells expressing markers associated with myeloid differentiation are decreased after induction of Gpr171 expression. (C) Representative flow plots showing decreased expression of two markers associated with terminal monocyte differentiation (F4/80 and CD16/32), following Gpr171 over-expression. Results are reported as mean ± SEM of at least triplicate experiments.