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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Cancer Res. 2012 Oct 22;72(24):6447–6456. doi: 10.1158/0008-5472.CAN-12-0193

Fig. 4. Treatment with shIDO-ST increases tumor influx of PMN and induces total intratumoral cell death.

Fig. 4

C57BL/6 mice bearing B16F10 tumors (≥7–8 mm diameter) were treated twice, 4d apart, with 2.5×106 shScr-ST, shIDO-ST, or PBS. Tumors were excised ~1 week after the 2nd treatment and single-cell suspensions were generated. A, Percentage of T, B, MDSC, and macrophage subsets from total intratumoral CD45+ cells using flow cytometry. B, Percentage of Ly6G+CD11b+ PMN gated from intratumoral CD45+ cells. C, left, Histogram from representative mouse of intratumoral CD45− Annexin V staining. right, Grouped (n=4) intratumoral Annexin V staining within CD45− population. D, left, Histogram from representative mouse of intratumoral CD45+ Annexin V staining. right, Grouped (n=4) intratumoral Annexin V staining within CD45+ population. ***P<0.001, by Student’s t test.