Figure 7. Disruption of Grx1 led to reduced neutrophil recruitment and impaired bacterial killing.

(A) Disruption of Grx1 led to reduced neutrophil accumulation in the inflamed peritoneal cavity. Data shown are mean ± SD of n=3 mice. *p < 0.01 by Student's t test. (B) Disruption of Grx1 led to impaired bacterial killing. Images of representative culture plates are shown. (C) Total numbers of survived E.coli in the inflamed peritoneal cavity. Data are means ± SD of 4 independent experiments. *p < 0.01 versus WT mice by Student's t test. (D) Schematic diagram of neutrophil adoptive transfer assay. Bone marrow neutrophils from WT and Grx1^−/− mice were labeled with different colors (SNARF1 or CFSE labeled), mixed 1:1 and then intravenously injected into WT recipient mice that have been challenged with E.coli (intraperitoneally injected). The relative recruitment of Grx1^−/− and WT neutrophils in the WT recipient was evaluated 60 min after the challenge. (E) The amount of adoptively transferred neutrophils recruited to the peritoneal cavity. Shown are representative FACS plots of an input control (left) and a mouse transplanted with labeled neutrophils (right). The double negative cells are the unlabeled endogenous cells. (F) Relative recruitment of neutrophil was calculated as the ratio of indicated populations in the peritoneal cavity. Results are the means (±SD) of three independent experiments. *, p < 0.01 versus WT neutrophils (Student’s t test).