Skip to main content
. 2013 Jan;93(1):135–143. doi: 10.1189/jlb.0112025

Figure 1. Analysis of 5LO synthesis and signaling cascades in the leukocytes of chimeric mice.

Figure 1.

(A) RT-PCR showing the expression of 5LO mRNA (band separated on a 1% agarose gel and visualized using 0.1% ethidium bromide) in circulating leukocytes. Lane 1: ND.WT; lane 2, SD.WT; lane 3, WT → ND.5LO−/−; lane 4, WT → SD.5LO−/−; lane 5, 5LO−/− → ND.WT; and lane 6, 5LO−/− → SD.WT. As anticipated, there is a lack of 5LO mRNA in mice transplanted with 5LO−/− bone marrow. (B) Western blot analysis of BLT1 expression in leukocytes from chimeric mice and control mice demonstrated that mice carrying 5LO−/− leukocytes lack a diabetes-induced rise in BLT1 expression. Data are representative of four different mice from each group. *, **P<0.05.