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. 2012 Dec 19;103(12):2521–2531. doi: 10.1016/j.bpj.2012.11.011

Figure 5.

Figure 5

pH dependence of the on-switching kinetics (trans-cis isomerization). (A) Thermally activated recovery of the anionic cis chromophore with rate coefficient, kth, measured at T = 290 K (open circles) and T = 310 K (solid circles). Stars, mIrisGFP Glu212Gln at T = 310 K; solid line, two-site protonation with pKa1 = 7.8 and pKa2 = 9.5; dotted line, one-site protonation with pK = 7.8; dashed line, one-site protonation with pK = 10.3. (B) Photoactivated trans-cis isomerization: kon(405) for P405 = 13 mW cm−2. Solid line shows one-site protonation with pK = 7.8.