Figure 6.
Action spectrum of cis-trans photoisomerization of mIrisGFP. The fluorescence decrease (symbols) at 520/540 nm due to photo-induced off-switching is plotted as a function of the wavelength. Also shown are the absorption (solid line), excitation (dotted line), and emission (dashed line) spectra at pH 5.3. Action spectrum and absorption amplitudes were normalized to 1 at the peak of the AC absorption band at 390 nm; the fluorescence spectra were normalized to match the peak of the BC absorption band.