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. 2012 Dec 19;103(12):2455–2464. doi: 10.1016/j.bpj.2012.11.004

Figure 2.

Figure 2

Self-association of GpA peptides in 20 mM lyso-PCs. Fluorescence emission was measured in micelles with Fl-labeled peptide alone as well as with the Fl- and TAMRA-labeled peptide pair (1:1 ratio). Energy transfer was calculated from the Fl-fluorescence decrease at 525 nm. (A) FRET spectra recorded for Fl- and TAMRA-labeled peptides dissolved in 20 mM Cn lyso-PC detergents. (Upper spectrum) This data originates from peptides dissolved in C10-lyso PC micelles and the others were measured in lyso-PCs having increasing acyl-chain lengths (C10–C16), resulting in decreasing energy transfer. (B) Fraction dimer plotted against the acyl-chain length of the various lyso-PCs.