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. 2012 Dec 19;103(12):2455–2464. doi: 10.1016/j.bpj.2012.11.004

Figure 3.

Figure 3

Concentration-dependent dimerization of the GpA TM domain. (A) GpA TM helix dimer fractions calculated from FRET spectra obtained at various C10 (●) and C16 (■) lyso-PC concentrations. The lyso-PC concentrations are given on the x axis. (Inset) FRET spectra recorded for Fl- and TAMRA-labeled peptides (1:1 mol ratio) in C10 lyso-PC micelles. All spectra were normalized at 525 nm. (Arrow) Spectral shifts at increasing C10 lyso-PC concentrations. (B) Apparent GpA TM dissociation constant determined in C10 (●) and C16 (■) lyso-PC micelles at increasing detergent concentrations. (C) Apparent dissociation free energy values (ΔG°) calculated from the apparent KD values shown in panel A and summarized in Table S1.