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. 2012 Dec 19;103(12):2455–2464. doi: 10.1016/j.bpj.2012.11.004

Figure 7.

Figure 7

FRET competition assay. (A) FRET pair emission spectra in 5 mM C12 lyso-PC. Addition of 0.4–2 μM unlabeled GpA TM peptide (compare panel A with panel B) results in reduced sensitized acceptor emission. Spectra were normalized at 525 nm. (Upper spectrum) This data originates from peptides in the absence of unlabeled peptide. Stepwise addition of increasing amounts of unlabeled peptide results in decreased energy transfer, i.e., in decreased fluorescence emission at 575 nm. (B) FRET efficiencies calculated from the spectra shown in panel A.