Figure 8.
Mechanism of action of β-amyrin-mediated MAGL inhibition. (A) Time course of MAGL inhibition by 3 µM β-amyrin. (B) Rapid dilution assays of MAGL in the presence of vehicle, β-amyrin or MAFP. Results are expressed as percentage of product generated after 60 min incubation with vehicle. (C) Michaelis–Menten analysis of the MAGL reaction (measured as pmol·min−1·µg protein−1) in the presence of vehicle or 3 µM of β-amyrin. Data show mean values ± SEM, n= 9 from three independent experiments.