Figure 3.

Direct inhibitory effect of AVX001, AVX002, AACOCF₃ and DHA on cPLA₂ activity in vitro. Recombinant cPLA₂ enzyme was incubated with either solvent (DMSO, final concentration 1%, 0) or the indicated concentrations of AVX001 (solid columns) or AVX002 (open columns), AACOCF₃ (hatched columns), and DHA (grey columns), and taken for an in vitro activity assay as described in the Methods section. cPLA₂ enzyme activity is given as % of control value (activity in the absence of inhibitors). The results shown are representative of at least three independent experiments, and data represent mean of duplicate determinations.