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. 2012 Nov 29;167(8):1691–1701. doi: 10.1111/j.1476-5381.2012.02114.x

Figure 6.

Figure 6

Effect of AVX inhibitors on IL-1β-stimulated NF-κB activation in mesangial cells. Quiescent cells were stimulated for 15 min with either vehicle (Co), or IL-1β (1 nM) in the absence (-) or presence of Bay11-7085 (Bay, 10 μM), AVX001 or AVX002 (both at 30 μM), pretreated for 90 min. Thereafter, cell lysates were separated by SDS-PAGE, transferred to nitrocellulose and subjected to a Western blot analysis using a polyclonal antibody against IκBα (inset, upper panel) and GAPDH (inset, lower panel). Bands corresponding to IκB and GAPDH were densitometrically evaluated and the ratio between IκB and GAPDH was calculated for each sample. Results are depicted as % of control stimulations and are means ± SD (n = 3). ***P < 0.001 considered statistically significant when compared with the control values; #P < 0.05, ##P < 0.01, ###P < 0.001 when compared with the IL-1β-stimulated values. The inset shows a representative experiment in duplicates.