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. 2012 Dec;10(4):198–207. doi: 10.1089/lrb.2012.0014

FIG. 6.

FIG. 6.

Lymphatic/blood endothelial cell connections during peak lymphatic sprouting. (A) Confocal projection of endothelial cell connections (arrows) between blood capillaries (BC) and lymphatic vessels (L) identified by PECAM immunolabeling at day 10. (B) Sub 0.5 μm confocal optical slice indicating continuous PECAM labeling along blood and lymphatic endothelial cell junctions (arrowheads). (C) Quantification of the number of apparent lymphatic/blood endothelial cell connections per overlapping blood and lymphatic vascular area. * represents a significant difference between groups (p<0.05). Values are means±SEM. (D–F) Intravenous injection of fixable FITC-dextran identified the lumens of blood vessels and capillary sprouts. The presence of dextran terminated along capillary sprouts proximal to endothelial cell connections (arrows). Scale bars=(A and B) 20 μm; (D–F) 50 μm.