FIG. 6.

Histologic analysis of constructs after subcutaneous transplantation. Dentin cylinders of group A are filled with only the hydrogel remnants and enclosed by a fibrous capsule (arrows) (A). In group B, the growth factors present in cell-free gels attract host cells from various tissues: brown adipose tissue (b), white adipose tissue with vacuoles (w), and fibrous tissue (f) (B). Group C displays DPSC embedded in the gel matrix (C). In group D, DPSC have formed a pulp-like soft connective tissue (D). Higher magnification shows blood vessels (bv) and a cell layer in intimate association with the dentin wall (E). The cells extend processes into the dentinal tubules (arrows), a characteristic of odontoblast cell morphology (F). The cell layer adjacent to the dentin as well as the cellular processes stain positive for dentin sialoprotein (G). Microvessels can be visualized after immunohistochemistry with Factor VIII–antibody (H). A–E: Hematoxylin and eosin. F: Masson's trichrome. G, H: Immunohistochemistry. d: dentin. Color images available online at www.liebertonline.com/tea