(Top panel) wild-type (A22678),
mad3∆ (A30386),
ndc80-1 (A28621),
ndc80-1 mad3∆ (A30390),
CUP-CLB3 (A22702),
CUP-CLB3 mad3∆ (A30388),
CUP-CLB3 ndc80-1 (A28623) and
CUP-CLB3 ndc80-1 mad3∆ (A30392) cells also carrying the
GAL4-ER and
GAL-NDT80 fusions were induced to sporulate at 25°C. 2 hr 45 min after transfer into sporulation medium CuSO
4 (50 μM) was added and concurrently, cultures were shifted to 36°C. Cells were subsequently released from
NDT80 block at 5 hr and transferred to 25°C. Percent binucleates with segregated heterozygous CENV-GFP dots was determined (n = 100). (Bottom panel) wild-type (A22688),
mad3∆ (A30638),
ndc80-1 (
A28625),
ndc80-1 mad3∆ (A30642),
CUP-CLB3 (A22708),
CUP-CLB3 mad3∆ (A30640),
CUP-CLB3 ndc80-1 (
A28627) and
CUP-CLB3 ndc80-1 mad3∆ (A30644) cells also carrying the
GAL4-ER and
GAL-NDT80 fusions were induced to sporulate at 25°C. 2 hr 45 min after transfer to sporulation medium CuSO
4 (50 μM) was added and concurrently, cultures were shifted to 36°C. After 5 hr, when cells had arrested in the
NDT80 block, cells were released and transferred to 25°C. The percentage of binucleate cells with segregated homozygous CENV-GFP dots was determined 7 hr 30 min after transfer into sporulation medium. Binucleate cells with GFP signal in only one of the two nuclei were categorized as having experienced a meiosis I non-disjunction event (n = 100). For top panel, using a chi-square test (df 1) the fraction of binucleates that underwent reductional or equational division was compared between
CUP-CLB3 and
CUP-CLB3 mad3∆ χ
2 = 0.1800, p=0.6714 and between
CUP-CLB3 ndc80-1 and
CUP-CLB3 ndc80-1 mad3∆ χ
2 = 0.02454, p=0.8755. For bottom panel, using a chi-square test (df 1) the fraction of binucleates that displayed MI nondisjunction or other was compared between
CUP-CLB3 and
CUP-CLB3 mad3∆ χ
2 = 1.228, p=0.2678 and between
CUP-CLB3 ndc80-1 and
CUP-CLB3 ndc80-1 mad3∆ χ
2 = 0.6486, p=0.4206.