Table 1.

Primer sequences used for the mutational analysis of β-catenin and APC genes in the Wnt pathway

Gene	Amplicon	Primer sequence	Amplicon size (bp)	Annealing temperature (°C)
β-catenin	Exon 3	BCat F: 5'-ATG GAA CCA GAC AGA AAA GC-3' BCat R: 5'-GCT ACT TGT TCT TGA GTG AAG-3'	200	58
APC	Exon 15	APC A F: 5'-CAGACTTATTGTGTAGAAGA- 3' APC A R: 5'-ATCCTGAAGAAAATTCAACA-3'	295 for codons 1260 to 1359	52
		APC B F: 5'-AGGGTTCTAGTTTATCTTCA-3' APC B R: 5'-TCTGCTTGGTGGCATGGTTT-3'	293 for codons 1339 to 1436	55
		APC C F: 5'-GGCATTATAAGCCCCAGTGA-3' APC C R: 5'-AAATGGCTCATCGAGGCTCA-3'	290 for codons 1417 to 1516	55
		APC D F: 5'-ACTCCAGATGGATTTTCTTG-3' APC D R: 5'-GGCTGGCTTTTTTGCTTTAC-3'	300 for codons 1497 to 1596	55