Fig. 3.

Effects of sesquiterpene fraction and the isolated sesquiterpenes on extracellular ACh concentrations in the nucleus accumbens. (A) Sesquiterpene fraction and vehicle, (B) isocomene, (C) β-isocomene, (D) modhephene, and (E) silphinene. Arrows indicate time when i.c.v. injection (20 μl at 2 μl/min) was started. Data are shown as relative values of basal release which is the mean of four pre-injection samples. Basal release is taken as 1.0 and indicated by a dotted line. *p < 0.05, **p < 0.01, Friedman’s analysis of variance followed by Dunn’s multiple comparisons test using the pre-injection sample as control.