FIG. 4.

DPP4 expression is increased on activation of inflammatory cells. A: T cells isolated from human peripheral blood were activated with anti-CD3 plus anti-CD28 mAb and then stained with IFN-γ and DPP4 Abs. IFN-γ--producing cells expressed higher levels of DPP4. Coexpression of DPP4 with IFN-γ (left) and DPP4 expression on IFN-γ--positive and IFN-γ--negative populations (right) are depicted. B: Peritoneal macrophages were collected from C57BL/6 mice and stained with CD11b, CD80, and DPP4 Abs. Activated macrophages (CD11b⁺ CD80⁺) expressed higher levels of DPP4. Coexpression of DPP4 with CD80 (left) on macrophages and the expression of DPP4 on CD80-positive and CD80-negative populations (right) are shown. C: Human peripheral blood mononuclear cells were stimulated with LPS (1μg/mL) for 24 h. DPP4 expression and activation were evaluated on CD14, CD11b, and CD11c populations. D: Peritoneal macrophages were isolated by injecting LPS (25 mg/kg body weight) intraperitoneally in C57BL/6 mice. Peritoneal macrophages were collected and DPP4 expression was evaluated on these cells. A higher maturation (bottom) and higher expression of DPP4 (top) was observed. (A high-quality color representation of this figure is available in the online issue.)