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. 2012 Dec 13;62(1):234–242. doi: 10.2337/db12-0161

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© 2013 by the American Diabetes Association.

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FIG. 2. — Confocal images indicating the proliferating cells for the pulsed hyperglycemic condition. The nuclei of the proliferating endocardial cells for the vehicle-treated (A), l-glucose–treated (B), and d-glucose–treated (C) OFTs are indicated by the green stain. Myocardial cell proliferation for vehicle (D), l-glucose (E), and d-glucose (F) is designated by the green nuclear stain (Alexa Fluor 488) with the red myosin stain (Alexa Fluor 564) to differentiate the cell type. Original magnification 400×. Scale bar represents 20 µm. The percentage of volumetric (G), endocardial cell (H), and myocardial cell (I) proliferation in the OFTs of HH24 embryos for pulsed additions of vehicle, l-glucose, and d-glucose are shown. Data are mean ± SD for n = 10.