FIG. 7.
In vitro assay using C2C12 cells. A: Real time PCR analysis of MEF2A and GLUT4 gene expression during differentiation of C2C12 cells from myoblast to myotubes with or without treatment of 10−8 mol/L angiotensin (Ang) 1–7; n = 4 in each group. *P < 0.05 vs. vehicle treatment control by a Student t test. B: Immunoblot analysis of MEF2A and GLUT4 protein levels 24 h after induction of differentiation of C2C12 cells with vehicle, 10−5 mol/L A779, 10−8 mol/L Ang 1–7, or Ang 1–7 with A779. Representative blot (left panel) and the relative intensities of GLUT4/α-tubulin and MEF2A/α-tubulin (right panel) are shown; n = 3 in each group. *P < 0.05 vs. vehicle by one-way ANOVA. C: Glucose uptake of C2C12 cells 24 h after induction of differentiation with or without treatment of 10−8 mol/L Ang 1–7; n = 3 in each group. *P < 0.05 vs. the same treatment without insulin by one-way ANOVA. †P < 0.05 vs. vehicle treatment with insulin by one-way ANOVA.