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. 2012 Dec 13;62(1):273–282. doi: 10.2337/db12-0172

FIG. 7.

FIG. 7.

Bone marrow neuropathy in Per2 mutant mice. Femurs collected from wild-type or Per2 mutant mice were decalcified paraffin embedded, sectioned on microtome at 4 μm, and stained for TH or NF-200. A: Representative images from TH-labeled bone marrow show TH expression near blood vessels (arrows). B: Per2 mutant bone marrow exhibited a dramatic reduction in TH labeling in 12-month-old animals, whereas an increase in TH expression in 4-month-old mice was found. C: Per2 mutant femurs were evaluated for bone marrow neural activity using NF-200 immunohistochemistry; photomicrographs show neurofilaments labeled with NF-200 (arrowheads) for 12-month-old mice. D: Quantification of NF-200 labeling showed a decrease in NF-200 expression in both 4- and 12-month-old mice. n = 7 for 4-month-old mice; n = 3 for 12-month-old mice; white bars, wild type; black bars, Per2 mutant.