FIG. 2.

Molecular response to a threefold increase in portal vein insulin infusion in animals characterized in Fig. 1. Hypothalamic insulin signaling indices (A) and hepatic insulin signaling indices (B). Hepatic gluconeogenic mRNA (C) and protein (D) expression. GK mRNA and protein expression (E); GSK3β mRNA, protein, and phosphorylation levels (F) and GS mRNA, protein, and phosphorylation levels (G) are shown. Representative Western blots can be found in Supplemental Fig. 4. Values are means ± SEM; n values are the same as in Fig. 1. *P < 0.05 difference versus the aCSF-treated hyperinsulinemic group.