Table 1.

Primer sequences for PKU exons 3, 6, 7, 11 and 12.

Exon	Sequences	Annealing temperature (°C)	Product length (bp)
3	F: 5′-GTTAGGTTTTCCTGTTCTGG-3′	60	300
	R: 5′-CTTATGTTGCAAAATTCCTC-3′
6	F: 5′-CACAGGTTCTGGTCCCCGAC-3′	61	354
	R: 5′-CTCTCCTCTCCTCAATCCTC-3′
7	F: 5′-CTCCTAGTGCCTCTGACTCA-3′	60	273
	R: 5′-CAAACCTCATTCTTGCAGCAGG-3′
11	F: 5′-AAGGAATCGGGGTGAGATGAGAGAAGGGGC-3′	64	357
	R: 5′-GGTACAAAGTTGCTGTAGACATTGGAGTCC-3′
12	F: 5′-ATGCCACTGAGAACTCTCTT-3′	60	245
	R: 5′-AGTCTTCGATTACTGAGAAA-3′