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. 2012 Nov 13;35(4):783–789. doi: 10.1590/S1415-47572012005000079

Table 2.

Specific primers used for real-time quantitative PCR (RT-qPCR)

Gene name Sequence Product size Position Accession number
β-actin F: 5′-GCCGCACCACTGGCATTGTC-3′ 399 bp Exon 2 DQ845171
R: 5′-AGGTAGTTTCGTGGATGCCGCAG-3′ Exon 3

SREBP-1c F: 5′-GCGACGGTGCCTCTGGTAGT-3′ 218 bp Exon 1 AY496867
R: 5′-CGCAAGACGGCGGATTTA-3′ Exon 2

H-FABP F: 5′-CCTGGAAGCTAGTGGACAGC-3′ 227 bp Exon 1 AJ416019
R: 5′-TGCCTCTTTCTCGTAAGTGC-3′ Exon 2

DGAT-1 F: 5′-AAGGACGGACACGACGATG-3′ 289 bp Exon 1 AY093657
R: 5′-GGAACGCAGTCACAGCAAAG-3′ Exon 5

LEPR F: 5′-GTGATAACTGCATTTGACTTGGC-3′ 285 bp Exon 1 NM001024587
R: 5′-CTGCAATGTTGTCTGCATGTACAG-3′ Exon 1

MC4R F: 5′-TGGAGAAAATCGCTGAGGCTACC-3′ 632 bp Exon1 NM214173
R: 5′-ATGATGAACAAAACACCCGACACC-3′ Exon2