Table 1.
Gene (accession no.) | Primers | Nucleotide sequence 5′-3′ | Size (bp) | Temp. (°C) |
---|---|---|---|---|
ACE | Forward | GTCCTATTCCCGCTCATCT | 128 | 53.1 |
Reverse | CCAGCCCTTCTGTACCATT | |||
| ||||
ACE2 | Forward | AGAATGCGACCATCAAGCG | 230 | 52.6 |
Reverse | AAGCCCAGAGCCTACGATT | |||
| ||||
GAPDH | Forward | CACTGCCACTCAGAAGACT | 177 | 53.5 |
Reverse | ACGTTGGGGGTAGGAACAC |