Table 1. Overview of incubation setup with single and multiple substrates, corresponding concentrations of cold substrates and final radioactivity in incubation vials.

Experiments with single substrates		Experiments with multiple substrates
Tracer	Substrate concentration and final radioactivity	Tracer	Substrate concentrationa and final radioactivity
3H-Acetate	4 mℳ, 20 μCi	3H-Acetate	Ace (4 mℳ, 20 μCi)+Prop (2 mℳ)+But (2 mℳ)+Val (1 mℳ)
14C-Propionate	2 mℳ, 10 μCi	14C-Propionate	Prop (2 mℳ, 10 μCi)+Ace (4 mℳ)+But (2 mℳ)+Val (1 mℳ)
14C-Butyrate	2 mℳ, 10 μCi	14C-Butyrate	But (2 mℳ, 10 μCi)+Ace (4 mℳ)+Prop (2 mℳ)+Val (1 mℳ)
14C-Valerate	1 mℳ, 5 μCi	14C-Valerate	Val (1 mℳ, 5 μCi)+Ace (4 mℳ)+Prop (2 mℳ)+But (2 mℳ)

^aAce, acetate; But, n-butyrate; Prop, propionate; Val, valerate.