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. Author manuscript; available in PMC: 2012 Dec 19.
Published in final edited form as: Science. 2012 May 25;336(6084):1040–1044. doi: 10.1126/science.1218595

Figure 1. Metabolite consumption and release (CORE) profiling.

Figure 1

A: For determining metabolite CORE (consumption and release rate) profiles, medium samples taken before (fresh) and after (spent) 4–5 days of cell culture are subjected to metabolite profiling using LC-MS/MS. For each metabolite X, the CORE value is calculated as the difference in molar abundance normalized to the area A under the growth curve. B: Glucose consumption vs. lactate release; glutamine consumption vs. glutamate release; and total measured carbon consumption vs. total measured carbon release, across the 60 cell lines. Gray lines indicate the 1:1 molar ratio of carbon consumed: carbon released for each metabolite pair; joined data points represent biological replicates.