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. 2012 Oct 11;40(22):11477–11489. doi: 10.1093/nar/gks926

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 6. — Relative expression levels of the neomycin resistance gene from the circular and linear HAC in the BALB/c and ES-R1 cells. NEO expression levels are normalized to the expression of housekeeping genes Hprt and Actb and are calculated as the relative expression level per L-HAC. The average expression level per background is depicted. Data are presented such that the average expression level of the L-HAC in the ES-R1 cells is 1. SDs from three independent clones for each background are shown.