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Human Rad18 is unaffected by fusion of SUMO to PCNA. In vitro ubiquitylation reactions were set up with human Rad6–Rad18 complex and human PCNA or a linear fusion with SUMO-2. Products were detected by western blot with an antibody against human PCNA. (A) Reactions set up without DNA and RFC. (B) Reactions set up in the presence of DNA and RFC.
