Figure 3.

Intrinsic Polµ binding to NHEJ substrates. (A) EMSA of Polµ (300 and 600 nM) using T/D substrates (formed by hybridization of GT and NHEJ-D oligonucleotides) either lacking (OH) or having (P) a phosphate group at the 5′-end of the downstream strand. The schemes show the free substrate (with a sphere depicting the 5′-P group) or the polymerase bound to the substrate. (B) Schematic representation of the possible arrangement of the three NHEJ substrates (the two DNA ends and the incoming nucleotide) mediated by Polµ.