Figure 1.

Validation of antisera against phosphorylated Hop1-T318 (A–C), Zip1-S75 (D) and Rad54-T132 (E). Western blot time course analyses of meiotic cells were performed as described previously (19,51). Wild-type and mutant strains at different sporulation time points are indicated. Anti-Hop1, anti-Zip1, anti-phospho-Hop1-T318, anti-phospho-Rad54-T132 and anti-phospho-Zip1-S75 antibodies were used to detect the corresponding proteins. Antibody against Hsp104 was used as a loading control. Molecular weights (in kiloDaltons) are indicated to the left of the blots.