Table 1.
β-galactosidase activities of wild type and ccpN knockout strains
| lacZ fusion | β-galactosidase activity (Miller units) in |
||||||
|---|---|---|---|---|---|---|---|
| DB104 (−Glc) | DB104 (+Glc) | GRF | ΔccpN (−Glc) | ΔccpN (+Glc) | GRF | F | |
| pACT87 Bsu | 2282 ± 171 | 205 ± 39 | 11.1 | 2181 ± 146 | 1453 ± 156 | 1.5 | 1.0 |
| pACS54 Bli | 1743 ± 34 | 129 ± 15 13 | 13.6 | 1687 ± 180 | 961 ± 95 | 1.8 | 1.0 |
| pACS55 Bam | 1567 ± 65 | 77 ± 19 | 20.4 | 1584 ± 111 | 983 ± 105 | 1.0 | 1.0 |
| pACS56 Bth | 4169 ± 88 | 428 ± 23 | 9.7 | 4224 ± 32 | 3736 ± 71 | 1.0 | 1.0 |
| pACS57 Bpu | 1785 ± 40 | 158 ± 23 | 11.3 | 2510 ± 139 | 2020 ± 195 | 1.2 | 1.4 |
| pACS58 Bha | 216 ± 19 | 22 ± 3 | 9.7 | 998 ± 127 | 526 ± 54 | 1.9 | 4.6 |
| pACS59 Bme | 223 ± 21,5 | 30 ± 14 | 7.4 | 809 ± 80 | 512 ± 76 | 1.6 | 3.6 |
| pACS60 Ban | 2341 ± 117 | 146 ± 22 | 16.3 | 2440 ± 126 | 2315 ± 38 | 1.1 | 1.0 |
| pACS61 Gka | 1714 ± 113 | 160 ± 36 | 10.7 | 1681 ± 106 | 1350 ± 21 | 1.2 | 1.0 |
| pACS62 Bsu C | 2300 ± 57 | 206 ± 11 | 11.2 | 2135 ± 18 | 1687 ± 76 | 1.3 | 0.9 |
| pACS63 Bsu P | 2196 ± 74 | 1590 ± 71 | 1.4 | 2042 ± 79 | 1496 ± 127 | 1.4 | 0.9 |
| pACS64 Bha C | 270 ± 14 | 58 ± 18 | 4.7 | 1100 ± 44 | 666 ± 10 | 1.7 | 4.0 |
| pACS65 Bha P | 1934 ± 1 | 1182 ± 166 | 1.7 | 1895 ± 47 | 1491 ± 26 | 1.3 | 1.3 |
| pACS66 Bme C | 322 ± 2.1 | 49 ± 34 | 6.7 | 1052 ± 62 | 522 ± 36 | 2.0 | 3.3 |
| pACS67 Bme P | 1736 ± 80 | 1582 ± 7 | 2.0 | 1571 ± 77 | 1611 ± 43 | 1.0 | 0.9 |
Bsu, B. subtilis; Bli, B. licheniformis; Bam, B. amyloliquefaciens; Bth, B. thuringiensis; Bpu, B. pumilus; Bha, B. halodurans; Bme, B. megaterium; Ban, B. anthracis; Gka, Geob. kaustophilus. All pAC derivatives contain the promoter, both CcpN sites and about 67 more upstream bp. Cultures were grown in SP medium with 2% or without glucose. All values represent averages of at least three independent determinations with five transformants grown in parallel; GRF, glucose repression factor, the ratio between the values obtained without and with glucose in wild-type DB104 and DB104 (ΔccpN::phleo), respectively. F, ratio of the values obtained for ΔccpN and DB104 without glucose. A value >1 suggests a glucose-independent repression by CcpN. Lower part: C, pAC derivative comprises promoter and both CcpN BS, but no further upstream sequences; P, pAC derivative contains only the promoter and CcpN BS II, but lacks BS I (Figure 2).