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. 2012 Oct 17;40(22):11435–11449. doi: 10.1093/nar/gks954

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 5. — Mre11 ATLD mutations partly destabilize MRN interactions in S. pombe. (A) Reciprocal co-IP of Nbs1-FLAG, Mre11-N122S-MYC and Mre11-W215C-MYC. Proteins were precipitated and detected with FLAG, MYC or tubulin antibodies. (B) Co-IP of TAP-Rad50 with Mre11-N122S and Mre11-W215C mutants. Proteins were pulled down with IgG and detected with PAP, MYC or tubulin antibodies. (C) Reciprocal co-IP of Nbs1-FLAG and Mre11-W248R-MYC. Proteins were precipitated and detected using FLAG, MYC or tubulin antibodies. (D) Co-IP of TAP-Rad50 and Mre11-W248R-MYC. Mre11-RRRR (CL454RR CV479RR) mutates Rad50 binding domain at C-terminus of Mre11 (HLH). Proteins were pulled down with IgG and detected with PAP, MYC or tubulin antibodies.