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. 2012 Oct 17;40(22):11435–11449. doi: 10.1093/nar/gks954

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© The Author(s) 2012. Published by Oxford University Press.

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Figure 6. — Mre11-W248R is proficient in Tel1 activation but partially defective for Ctp1 recruitment. (A) Immunoblot of phosphorylated histone H2A (γH2A) and total H2A with or without treatment with 90 Gy of IR. Graph represents γH2A expression relative to total H2A with error bars representing standard deviation from the mean of three independent experiments. (B) γH2A levels induced specifically by IR treatment determined by subtracting basal levels of γH2A from IR-treated shown in Panel A. (C) Southern blot probing for telomere-associated sequences (TAS1) from genomic DNA isolated from indicated genetic backgrounds and digested with EcoRI (top panel). Ethidium bromide (EtBr)-stained gel shows DNA loading (bottom panel). (D) Chromatin immunoprecipitation of Ctp1-TAP to an HO-induced DNA double-strand break in mre11⁺ and mre11-W248R backgrounds. Schematic of chromosome I containing HO break site and the relative distances and expected DNA sizes assayed by multiplex PCR is shown on top.