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. 2012 Oct 12;40(22):11618–11626. doi: 10.1093/nar/gks948

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© The Author(s) 2012. Published by Oxford University Press.

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Figure 3. — PCNA-mediated translesion activity of Pol ζ₂ and Pol ζ₄. (A) A schematic diagram of the oligonucleotide substrate. The template is a 102-mer with streptavidin-biotin blocks at the 5′ and 3′ ends. The template at the +2 position is either a C (in (B)) or an abasic site, indicated as a ‘0’ (in (C) and (D)). The 72-mer products represent full extension of the 30-mer primer to the end of the template. PCNA (30 nM) was added where indicated in (B–D). See Materials and Methods for details. (B) Time course of reactions of Pol ζ₂ and Pol ζ₄ on undamaged template DNA. (C) Time course of translesion synthesis by Pol ζ₂ and Pol ζ₄ on an abasic site (0) template. (D) Stimulation by PCNA of the DNA polymerase activity of Pol ζ₄, Pol ζ₄-ΔPIP and Pol ζ₄-CysA on template DNA with an abasic site. Asterisk indicates an impurity in the radiolabeled primer.