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. 2012 Oct 12;40(22):11618–11626. doi: 10.1093/nar/gks948

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© The Author(s) 2012. Published by Oxford University Press.

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Figure 4. — Stability of Pol ζ₄ during the cell cycle. (A) Fluorescence-activated cell sorting analysis of the DNA content of cells. Cells expressing low levels of GST-REV3 and REV7, and POL31 and POL32 at native levels, were arrested in G1, S, or G2 phase, followed by treatment with MMS or 4NQO. (B) Extracts were prepared from arrested cells, and Pol32, Rev7 and Rev1 were detected by western analysis after GST-Rev3 pull down with glutathione sepharose beads. Control: Western analysis of extracts made from cells lacking GST-Rev3 and subjected to glutathione affinity purification.