. 2012 Oct 18;40(22):11352–11362. doi: 10.1093/nar/gks973

Table 1.

Dissociation constants for the binding of promoter fragments to Taq Eσ^A and Ec Eσ⁷⁰ at 25°C

Promoter fragments	DNA probe	K_d^taq, nM	K_d^ec, nM	K_d^taq/K_d^ec
Oligos	1. −12/+2	100	160	0.63
	3. −12/+2; ggga	15	110	0.14
	4. −12/+2; ccct	2200	690	3.2
	5. −12/−6	54000	80000	0.68
Upstream fork junctions	6. [−38/−11][−38/−12]	13.7	5	2.8
	7. [−38/−11][−38/−12]TG	1.2	0.43	2.8
	8. [−38/−8][−38/−12]	0.8	0.14	5.7
	9. [−38/−7][−38/−12]	<0.2	<0.2
	10. [−38/−7][−38/−12] −35mut	0.57	2.1	0.27
	11. [−26/−3][−26/−12]	0.65	0.46	1.4
	12. [−59/−11][−59/−12]UPec	4.1	0.008	510
	13. [−59/−11][−59/−12]UPth	3.4	0.35	9.7
Downstream fork junctions	14. [−12/+20][+3/+20]	1.6	<0.2	>8
	15. [−12/+20][+3/+20]ggga	0.2	<0.2
	16. [−11/+14][+2/+14]	42	0.25	170

The K_d values presented are averages obtained from two to three individual experiments, the error is ±15%.