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. 2012 Aug 13;40(22):e170. doi: 10.1093/nar/gks751

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — DnaG variants improve MAGE performance. EcNR2 (wt) and EcNR2.dnaG.Q576A (Q576A) were tested for their MAGE performance without co-selection (4) using 3 sets of 10 oligos as described in Figure 1B. For each set, all 10 alleles were simultaneously assayed by mascPCR after one cycle of MAGE. The data are presented using stacked AR frequency plots, which show the distribution of clones exhibiting a given number of allele conversions. Compared with EcNR2 (A, Set 1, n = 69; B, Set 2, n = 47; C, Set 3, n = 96), EcNR2.dnaG.Q576A exhibited fewer clones with zero conversions for Set 1 (A, n = 90) and Set 2 (B, n = 46), but not for Set 3 (C, n = 96). In all three sets, EcNR2.dnaG.Q576A displayed more clones with 2 or more allele conversions.