Table 2.

CoS-MAGE AR performance of modified strains (presented as fold change from EcNR2)

Metric	Set	Nuc5-	E2.dnaG. Q576A	Nuc5-.dnaG. Q576A
Average	1	1.65	1.49	2.40
	2	1.41	1.29	1.82
	3	1.32	2.08	2.12
	Average	1.46	1.62	2.11
5+ Conversions	1	5.28	3.96	10.18
	2	2.65	2.01	4.11
	3	1.07	4.20	4.52
	Average	3.00	3.39	6.27
0 Conversions	1	0.67	0.68	0.24
	2	0.58	0.79	0.35
	3	0.71	0.40	0.30
	Average	0.65	0.62	0.29

The fold improvement was calculated as (strain performance)/(EcNR2 performance), where performance refers to the average number of allele conversions per clone, or the fraction of clones with 5+ or 0 conversions. These metrics were the average of individual metrics for Oligo Sets 1, 2 and 3. In all three categories, Nuc5-.dnaG.Q576A exhibited an effect that was roughly an additive combination of the effects yielded in Nuc5- and EcNR2.dnaG.Q576A. Data for EcNR2.dnaG.Q576A and Nuc5-.dnaG.Q576A were determined in this work. Data for EcNR2 and Nuc5- are from Mosberg, J.A., Gregg, C.J., et al. (in review).