Figure 4.

AUF1 regulates Dicer expression through the DICER1 CR. (A) HeLa cells were transfected with the plasmids and siRNAs shown; 48 h later, the levels of Dicer, AUF1 and β-Actin were assessed by western blot analysis. Dicer signals were quantified by densitometry and represented as a percentage of Dicer levels in the control group. (B and C) HeLa cells were transfected with a plasmid that expressed the Dicer CR (pcDNA-Dicer) and the endogenous Dicer was silenced using a DICER1 3′UTR-directed siRNA. The consequences of AUF1 overexpression on Dicer protein expression levels were assessed by western blot analysis (B), and the consequences on DICER1 mRNA levels were measured by RT-qPCR analysis (C). Data in panel (C) represent the means (±SD) of three independent experiments.