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. 2012 Oct 5;40(22):11240–11255. doi: 10.1093/nar/gks873

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — AS events in AtGRP7-ox lines not observed in AtGRP7-R⁴⁹Q-ox plants. Left side: RT-PCR analysis of selected genes/transcripts with total RNA from AtGRP7-ox and AtGRP7-RQ-ox plants in both the Col-2 and C24 backgrounds and their respective wt plants. Arrowheads denote RT-PCR products representing AS events whose presence/absence or abundance differs between the over-expression lines with and without the R⁴⁹Q mutation. Right side: gene and transcript structures and the AS events for AtGRP8 (#90), ADA2A (#136), Aly/Ref (#327), AKIN11 (#343) and AFC2 (#226) are shown schematically (see Figure 2). PP2A served as constitutive control.