Skip to main content
. 2012 Oct 5;40(22):11684–11696. doi: 10.1093/nar/gks912

Figure 2.

Figure 2.

Expression of human miRNAs suppress HIV-1 replication in cell culture. (A) Effects of transfection of the indicated individual miRNAs on HIV-1 replication in cultured cells. The 42CD4 cells were transfected with the indicated miRNAs, and cells were infected with HIV-1 NL4-3 24 h later. Viral replication was monitored by measuring RT production in culture supernatants, 3 days after infection. Values are expressed as percent of a control irrelevant RNA oligonucleotide (oligo fluo). Arrows indicate miRNA chosen for follow-up experiments. (B) Time course replication curves spanning 2, 3, 4 and 6 days post-infection in cells transfected with miR-138, miR-149, miR-326, miR-195 and miR-29b (indicated by an arrow in panel A) and irrelevant control RNA oligonucleotide (oligo fluo); miR-29b is a positive control; oligo fluo is a negative control. Results are representative of three independent experiments. Values are the mean of three separate experiments with standard deviations.