Skip to main content
. 2012 Oct 5;40(22):11684–11696. doi: 10.1093/nar/gks912

Figure 5.

Figure 5.

Increased miRNA–mRNA complementarity correlates with greater reduction of expression in reporter assays. (A) Schematic representation of CMV promoter-driven reporter constructs engineered to contain three tandem copies of either the Wt or Tgt target sites positioned downstream of the luciferase gene. (B) Effect of miR-326 on the Wt and Tgt target sites. The 293T cells were transfected with Luc-3X Wt or Luc-3X Tgt together with an internal control pMIR-βgal plasmid. After 24 h post -transfection, cells were lysed and luciferase and βgal activities were measured. The luciferase activity from Luc-3X Tgt, after normalization to βgal, was expressed relative to that from the Luc-3X Wt construct. (C) Effect of transfected miR-326 on Luc-3X Wt and Luc-3X Tgt. miR-326 (+) or negative control (−) siRNA (negative control siRNA #1, Ambion) was transfected into 293 T cells with either the Luc-3X Wt or Luc-3X Tgt constructs and the internal control pMIR-βGal. Luciferase and βgal activities were monitored 24 h post-transfection. Values (+) are given as percent of the value from the negative control siRNA (−) transfected cells which was set to 100%. Values are the mean of three separate experiments presented with standard deviations.