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. 2012 Dec 17;209(13):2351–2365. doi: 10.1084/jem.20120944

Figure 5.

Figure 5.

The rapid proliferation of IL-12/15/18–preactivated NK cells is dependent on CD4+ T–producing IL-2. CFSE-labeled IL-12/15/18–pretreated NK (CD45.1+) cells were transferred into RMA-S tumor–bearing, irradiated mice (CD45.2+) as described in Fig. 2 b. Anti-CD4 or anti-CD8 mAb was applied as described in Materials and methods. 4 d later, spleen cells were analyzed. (a) Spleen cells were restimulated by PMA/ionomycin, and IL-2 expression was determined by intracellular staining. One representative dot plot gated on total splenocytes is shown. Numbers indicate percentages among total splenocytes. Percentages of IL-2–producing CD4+ or CD4− cells (after subtraction of the isotype control) in spleen are shown in the graph (mean + SD; n = 3). n.d., not detectable. Data are representative of three independent experiments. (b) CFSE-dilution in spleen was determined. Data shown were gated on CD3ε−NK1.1+CD45.1+ transferred NK cells, and one representative histogram from each group is shown (n = 3). The replication index of transferred NK cells was calculated by FlowJo. Numbers of transferred NK cells in spleen are shown. Graphs indicate mean + SD (n = 3). Data are representative of three independent experiments.