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. 2012 Dec 17;209(13):2351–2365. doi: 10.1084/jem.20120944

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© 2012 Ni et al.

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Figure 6. — IL-12/15/18–preactivated NK cells display a mature phenotype before and after transfer. NK cells (CD45.1⁺) were treated with IL-15 or IL-12/15/18 for 16 h and transferred into tumor-bearing, irradiated mice (CD45.2⁺; n = 5) as described in Fig. 2 b. (a) Dot plots show the expression of CD11b and CD27 on splenic naive NK cells (gated on CD3ε⁻NK1.1⁺) and IL-15– or IL-12/15/18–preactivated NK cells before (gated on CD3ε⁻NK1.1⁺) and after transfer (gated on CD3ε⁻NK1.1⁺CD45.1⁺ transferred NK cells) on day 11. Numbers indicate percentages. (b) Histograms show expression of KLRG1 and CD43 on NK cells. Numbers indicate geometric mean fluorescence. All data are representative of two independent experiments.