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. 2012 Oct 30;9:247. doi: 10.1186/1742-2094-9-247

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Copyright ©2012 Ziebell et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Neuronal processes are the tracks for rod microglial trains. Double-labeling of microglia (Iba1; red) with neuronal structural markers (green) and Hoechst nuclear dye (blue). (A) Ramified microglia (Iba1; red) in uninjured sham brain showed no association with MAP-2, neurofilament, or the pan-neuronal marker. Rod microglia in the sensory cortex 7 days post-injury were in close apposition with dendrites (MAP-2) and axons (neurofilament). (B) Additional staining with a pan neuronal marker clearly demonstrated the proximity of trains of rod microglia to neuronal structures via confocal analysis.