Table 2. Standard setup and Ct range for qPCR reactions.

16S	Standard type	Standard range	Ct value range	NTC Cta
Bacteriab	Amplicon	2.89×103 to 2.89×107	9.50±0.49 to 24.48±0.06	≥35
	Linear	2.56×103 to 2.56×107	10.39±0.14 to 25.35±0.11	≥35
	Nicked circles	2.04×103 to 2.04×107	11.31±0.03 to 25.68±0.17	≥35
	Supercoiled	2.40×103 to 2.40×107	11.53±0.07 to 26.17±0.03	≥35
Archaeac	Amplicon	4.25×102 to 4.25×107	9.18±0.10 to 27.43±0.17	≥35
	Linear	3.28×102 to 3.28×107	10.54±0.02 to 28.46±0.01	≥35
	Nicked circles	2.52×102 to 2.52×107	11.27±0.15 to 28.52±0.15	≥35
	Supercoiled	4.99×102 to 4.99×107	9.74±0.19 to 27.39±0.11	≥35

^aCt value for the NTC (no template control).

^bBacteria: T. lienii.

^cArchaea: A. fulgidus.